WebJul 1, 2024 · Yes, Cy5 is strongly hydrophobic. Cy5 is a commonly used far-red fluorophore. It fluoresces at ~650 nm excitation and ~670 nm emission and is used in a wide range of … WebAre compatible with all common excitation sources ( Table 2) and instruments Can replace standard fluorophores for most applications ( Table 3) Produce the brightest and most photostable conjugates, for …

Cy5 biotin conjugate AAT Bioquest

WebA single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection … WebThe fluorescence emission spectra of Cyanine5, Sulfo-Cyanine5, BDP 630/650 NHS esters. Similarly, you can see from the graphs and a figure that the fluorescence intensity (RFU) of the cyanine dyes Cyanine5 and Sulfo-Cyanine5 is independent of the pH and remains nearly constant (within the range 5%). debo spanishdict

Does the fluorescence of cyanine dyes depend on pH?

WebAug 8, 2009 · The pH low insertion peptide (pHLIP) is a peptide of 37 amino acids that inserts across the cell membrane as an α-helix when the extracellular pH (pHe) is acidic … WebA mixture of proteins labelled with Cy5. Positive control IHC-Free Floating: Rat brain tissue. Flow Cytometry: Purified human CD4+ cells. General notes Monoclonal antibodies to Cy5 are an important tool for Cy5 signal amplification in assays … WebCy5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin. Platform Other instruments Example protocol Calculators Common stock solution preparation Table 1. de boshut hilversum