WebLuciferase-silencing efficiency was calculated relative to untreated cells. While GenMute™ and Dharmafect™ 4 reagents delivered significant gene silencing from 1.0 nM of renilla luciferase siRNA, li pofectamine™ 2000 gave good … WebDharmaFECT-4 0.75mL T-2004-02 $209.00 DharmaFECT Set 4 x 0.75mL T-2005-02 $790.00 (Reagents 1-4) Mirus Bio™ TransIT-TKO™ siRNA Transfection Reagent A high efficiency, low toxicity, siRNA transfection reagent for mammalian cells.
RNA—Purification Through Analysis - Fisher Sci
WebAt the same time, Lipofectamin, Dharmafect 1, Dharmafect 2, Dharmafect 3 and Dharmafect 4 were complexed with the pCMV-EGFP plasmid at the reagent: DNA ratio respectively of 4 µl:1.5 µg for Lipofectamin and 2.5 µl:1.5 µg for the 4 Dharmafect reagents. Complexes were prepared by mixing the appropriate amount of lipofection reagent … WebThe authors report the optimization of transfection conditions for Raw 267.4 macrophage cells. The herein described proce-dure makes use of automated confocal microscopy, enhanced green fluorescent protein (EGFP)–expressing macrophages, ... Dharmafect 1 (cat. T2001-02), Dharmafect 2 (cat. T2002-02), Dharmafect 3 (cat. T2003-02), and ... date range search windows 10
Dharmacon™ DharmaFECT™ Transfection Reagent …
WebmiR control or human pre-miR-142-3p (12.5 nM) using DharmaFECT 4 transfection reagent (T-2004-03, Thermo Scientific) and analyzed after 48h. Transfection of miR inhibitors: 250,000 cells/ml were transfected with inhibitor control or miR-142-3p inhibitor (20 nM) using DharmaFECT 4 transfection reagent (T-2004- WebThermo Scientific Dharmafect Transfection Reagents - Fisher Sci WebDharmaFECT 4 Normalized Values (%) 140 120 100 80 60 40 20 0 Target mRNA levels Cell viability Optimal transfection conditions. co-transfect with confidence DharmaFECT Duo transfection reagent is specially formulated to provide: High co-transfection efficiency of plasmid and siRNA or miRNA reagents bizspace business park tyseley