Fischer rat thyroid frt cells
WebMay 21, 2024 · Fisher Rat Thyroid (FRT) cells stably transfected with a wild type (WT) CFTR, or CFTR carrying the cystic fibrosis mutation p.F508del, were grown at 37 °C and 5% CO 2 in modified F12 Coon’s ... WebThe effects of ivacaftor on CFTR channel open probability and chloride transport were tested in electrophysiological studies using Fischer rat thyroid (FRT) cells expressing different CFTR gating ...
Fischer rat thyroid frt cells
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WebJun 27, 2024 · Fischer rat thyroid (FRT) cells were used as a cell model of a polarized epithelium (Zurzolo et al., 1991). A stable FRT cell line was generated by transfecting in the bicistronic plasmid expressing both KCa3.1-BLAP and BirA-KDEL into FRT cells using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions and selecting a ... WebJan 12, 2024 · Fisher rat thyroid (FRT) cells were cultured in DMEM/F-12 medium (plus 8% FBS) and transiently transfected with plasmids (0.5 µg per construct) using Lipofectamine 3000 (Invitrogen, L3000008) according to the manufacturer’s instruction. An equal amount of a mouse PON2 cDNA or the pCMV6 vector was cotransfected with …
WebOct 13, 2024 · The MAL proteolipid has been recently demonstrated as being necessary for correct apical sorting of the transmembrane influenza virus hemagglutinin (HA) in Madin–Darby canine kidney (MDCK) cells. The fact that, in contrast to MDCK cells, Fischer rat thyroid (FRT) cells target the majority of glycosylphosphatidylinositol (GPI) … WebTo give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy. GROW. Gibco cell culture media & reagents.
WebMar 5, 2001 · Fischer rat thyroid (FRT) cells (Lipardi et al. 1998), the gift of Dr. Enrique Rodriguez-Boulan (Cornell University, Ithaca, New York), were maintained in Coon's F12 media with 10% FBS. COS, CHO, and HEK 293 cells (American Type Culture Collection) were maintained in DME with 5% iron-supplemented calf serum or 10% FBS. WebPMID: 9709917. DOI: 10.1089/thy.1998.8.623. Abstract. The FRTL-5 cell line is a stable thyroid cell line derived from the thyroid gland of the Fischer rat under defined …
WebMay 7, 2024 · The membrane protein Ano1 and the intracytoplasmic yellow fluorescent protein mutant (YFP-H148Q/I152L) were overexpressed in Fischer rat thyroid (FRT) cells that endogenously express P2Y2 receptors. Ano1, a calcium-activated anion channel, is a powerful transporter of iodide ions, where a single channel is capable of reaching 10 6 …
WebSep 8, 2015 · Fisher rat thyroid (FRT) epithelial cells as a model as they have been shown to be suitable for studies of other epithelial ion channels including CFTR and epithelial Na channels at the level of single cells, as well as cell monolayers suitable for integrated studies of transepithelial ion transport (32, 33). FRT cswd cavite cityWebJun 7, 2024 · Treatment with methyl-β-cyclodextrin (MβCD) reduces total cholesterol level in Fischer rat thyroid (FRT) cells. (A) A representative extracted ion chromatogram for m/z 369.3513 (cholesterol) from mass … earnhardt buick gmc incWebOct 13, 2024 · Fischer rat thyroid (FRT) cells are the only known epithelial cells that sort most of their endogenous GPI-anchored proteins to the basolateral surface (Zurzolo et al., 1993). Although they have TX … cswd cash assistanceWebWe would like to show you a description here but the site won’t allow us. cswd chuck it guideWebNov 29, 2024 · After extensive experiments, we found that the M3 receptor is endogenously expressed in Fischer rat thyroid (FRT) cells. FRT cells can be grown on relatively inexpensive media with good adhesion to commonly used cell culture plates while allowing stable structures expression of the transfected protein. Therefore, we applied FRT cells … cswd batangas cityWebApr 20, 2024 · Here we used Fisher rat thyroid (FRT) cells stably transduced with rare human CFTR mutants and studied their responsiveness to the dual phosphodiesterase 3/4 inhibitor RPL554 (Verona Pharma). Through its inhibitory effect on PDE4D, we find that RPL554 can elevate intracellular cAMP leading to a potentiation of forskolin-stimulated … earnhardt buick gmc mesa azWebSep 8, 2009 · functional and biochemical assays on two different cell expression systems, Fischer rat thyroid (FRT) and A549 cells. The order of potency and efficacy of potentiators was similar in the two cell types considered, with phenylglycine PG-01 and isoxazole UCCF-152 be-ing the most potent and least potent, respectively. Most … cswd burlington