WebHieff NGS™ Ultima DNA Library Prep Kit for MGI® is compatible with DNA fragmented by mechanical methods and enzyme digestion methods. CN EN ... EN-13310 Hieff … Web24 de ago. de 2024 · The PCR products were purified with the DNA Clean-Up Kit (Axygen), then Illumina barcodes and adapter sequences were added via PCR, after which the resulting library was separated on a 1% agarose gel. DNA in a 100–400 bp size range was selected and isolated using the Hieff NGSTM DNA Selection Beads Kit (Yeasen).
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Web产物如需纯化，可使用 Hieff NGS ® DNA Selection Beads （ Cat#12601 ）或 AMPure XP Beads （ Cat#A63880 ）或其他等效产 品。 参考实例 使用 Hieff ® Smearase 对 Human gDNA 样本进行酶切，片段化结果见图 1 。 WebFigure 3. DNA purification steps. Figure 4. DNA size selection steps. 1.3 Tips for using magnetic beads. Improved yield and accurate size selection (1) Mix well before use, and equilibrate the magnetic beads to room temperature for at least 30min. ——The solubility of PEG in the magnetic beads buffer is easily affected by pH and temperature. small plastic baseball bats
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WebHieff NGS™ Ultima Dual-mode RNA Library Prep Kit for Illumina™ is an RNA sequencing library construction kit for the Illumina™ sequencing platform, including RNA fragmentation reagents, reverse transcription reagents, conventional and strand-specific ds-cDNA synthesis reagents, and library amplification reagents. http://www.bioon.com.cn/doc/showarticle.asp?newsid=68005 Web6 de mar. de 2024 · 如需进行片段分选，则进行以下步骤，推荐使用Cat#12601 DNA Selection Beads进行产物片段分选，为防止接头或大片段残留，可进行两次片段分选，或先使用1.0×磁珠纯化扩增产物后再进行分选。 1. 将平衡至室温的Hieff NGS TM DNA Selection Beads磁珠，振荡或上下颠倒混匀。 2. small plastic banks